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KMID : 1377020150120060435
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Tissue Engineering and Regenerative Medicine
2015 Volume.12 No. 6 p.435 ~ p.441
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Effects of adipose-derived stem cells on keloid fibroblasts based on paracrine function
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Kim Sang-Wha
Kim Ki-Joo
Rhie Jong-Won
Ahn Sang-Tae
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Abstract
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Adipose-derived stem cells (ASCs) possess multipotent differentiation capabilities, including the ability to repair and regenerate injured tissues. Recent studies have reported that ASCs have effects on fibroblasts that are related to wound healing. On the other hand, keloid is a result of pathologic wound healing, and keloid fibroblasts are known to have higher growth potential and an abnormal balance between proliferation and apoptosis. This study aimed to explore the paracrine effects of ASCs on normal fibroblasts (NF) and keloid fibroblasts (KF). NF and KF were cultured alone and in combination with ASCs using a trans-well cell-contact-independent cell culture system. Cell morphology was observed, cell proliferation was assessed using a CCK-8 kit, and apoptosis was assessed by live-dead-cell staining on days 1, 3, and 7. Western blot analysis at 24 hours after culture was used to investigate the production of type 1 collagen and alphasmooth muscle actin (α-SMA). Co-culture with ASCs resulted in increased proliferation of both NF and KF. In the co-culture with ASCs, collagen production decreased and expression of α-SMA increased in both the NF and KF. Comparison between the four groups revealed a significant difference in the expression of both type I collagen and α-SMA (p=0.016 and 0.022; Kruskal-Wallis test). Our findings are different from previous studies that used ASCs for normal dermal fibroblast and normal wound healing. However, considering pathologic mechanisms of keloid, ASCs may have the different effect on keloid fibroblasts, which could be another effect of ASCs modulating keloid during healing process.
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KEYWORD
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Adipose-derived stem cells, Fibroblast, Co-culture, Keloid
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